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tagA [2019-08-12 08:49:57]
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tagA [2019-08-12 08:49:57]

UDP-N-acetyl-D-mannosamine transferase
locus
BSU_35750
pI
10.13
mw
29.35 kDa
protein length
256 aa Sequence Blast
gene length
771 bp Sequence Blast
function
biosynthesis of teichoic acid
product
UDP-N-acetyl-D-mannosamine transferase
essential
yes
ec
2.4.1.187
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.4|Biosynthesis of teichoic acid]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.3|Biosynthesis of teichoic acid]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    3,681,370 3,682,140

    Phenotypes of a mutant

  • essential [Pubmed|12682299], reduced growth rate, loss of rod-like morphology [Pubmed|19376878]
  • inactivation of ''[gene|72A89412C53703F96F835D26307263F5D1A4AB4E|tagA]'' strongly restores beta-lactam resistance in a ''[gene|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]'' mutant [Pubmed|22211522]

    • The protein

    Catalyzed reaction/ biological activity

  • UDP-N-acetyl-D-mannosamine N-acetyl-D-glucosaminyldiphosphoundecaprenol = UDP N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol (according to Swiss-Prot)

    • Protein family

  • glycosyltransferase 26 family (single member, according to UniProt)

    • Structure

  • [PDB|5WB4] (from Thermoanaerobacter italicus, corresponds to aa 35 ... 194, 33% identity)

    • [SW|Localization]

  • secreted (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|72A89412C53703F96F835D26307263F5D1A4AB4E|tagA]-[gene|911A4D957A6C0DFC636A152395119DBFC777DDA2|tagB]
    description
    [Pubmed|7952180]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7581998], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: repression, [Pubmed|9457886], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]
  • [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]: activation, [Pubmed|12950927], in [regulon|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR regulon]

    • regulation

  • repressed when phosphate is available ([protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]) [Pubmed|9457886]
  • the leader mRNA is processed upstream of [gene|72A89412C53703F96F835D26307263F5D1A4AB4E|tagA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]
    • view in new tab

    References

    Reviews

  • 24024634

    • Original publications

  • 18156271,9457886,9683503,7952180,7581998,19376878,12950927,22211522,25666134,29794222